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ecori sites
Ecori Sites, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 15375 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ecori+sites/EcoRI/pmc13170675-222-24-29
Average 99 stars, based on 15375 article reviews
Ecori Sites, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 15375 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ecori+sites/EcoRI/pmc13170675-222-24-29
Average 99 stars, based on 15375 article reviews
ecori sites - by Bioz Stars,
2026-09
99/100 stars
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Polymerase Chain Reaction:Article Title: The Yersinia pestis virulence effector YopM binds to a key regulatory site on the human pyrin death domain to inhibit inflammasome activation and effector-triggered immunity Article Snippet: .. All inserts were first PCR amplified then inserted into their respective vectors by either restriction cloning using BamHI and Article Title: A Wnt-induced conformational phospho-switch in DVL3 controls association with Frizzled receptors and Wnt/β-catenin signaling Article Snippet: hDVL3 segments of interest (IDR2, amino acids 335 to 396; DEP, amino acids 398 to 496; PDZ, amino acids 243 to 351; and PDZ-IDR2-DEP, amino acids 243 to 496) were amplified by polymerase chain reaction (PCR) and inserted into a pETM11 or pET-Z (IDR2) expression vectors via restriction digestion between the 5′-NcoI and 3′-KpnI sites, using BspHI (R0517, NEB) and KpnI (R3142S, NEB) restriction enzymes. .. The DNA segment encoding CK1δ or ε was amplified by PCR and inserted into pETM11 expression vector via restriction digestion between the 5′-NcoI and Amplification:Article Title: The Yersinia pestis virulence effector YopM binds to a key regulatory site on the human pyrin death domain to inhibit inflammasome activation and effector-triggered immunity Article Snippet: .. All inserts were first PCR amplified then inserted into their respective vectors by either restriction cloning using BamHI and Article Title: A Wnt-induced conformational phospho-switch in DVL3 controls association with Frizzled receptors and Wnt/β-catenin signaling Article Snippet: hDVL3 segments of interest (IDR2, amino acids 335 to 396; DEP, amino acids 398 to 496; PDZ, amino acids 243 to 351; and PDZ-IDR2-DEP, amino acids 243 to 496) were amplified by polymerase chain reaction (PCR) and inserted into a pETM11 or pET-Z (IDR2) expression vectors via restriction digestion between the 5′-NcoI and 3′-KpnI sites, using BspHI (R0517, NEB) and KpnI (R3142S, NEB) restriction enzymes. .. The DNA segment encoding CK1δ or ε was amplified by PCR and inserted into pETM11 expression vector via restriction digestion between the 5′-NcoI and Article Title: A combinatorial genetics approach reveals limits to redundancy within Plasmodium falciparum invasion ligand families Article Snippet: .. Amplified homology regions and recoded repair templates were inserted into the pCC1 plasmid (PlasmoGEM Sanger) between SpeI and Cloning:Article Title: The Yersinia pestis virulence effector YopM binds to a key regulatory site on the human pyrin death domain to inhibit inflammasome activation and effector-triggered immunity Article Snippet: .. All inserts were first PCR amplified then inserted into their respective vectors by either restriction cloning using BamHI and Construct:Article Title: METTL1 interacts with XPO5 to modulate pre-miRNA export. Article Snippet: The cells were verified to be free from mycoplasma contamination using the LookOut Mycoplasma PCR Detection Kit (MP0035, Sigma–Aldrich). .. The METTL1-V5-APEX, EGFP-V5-APEX, and APEX2-NLS plasmids were constructed by inserting the coding sequence of full-length METTL1, enhanced green fluorescent protein (EGFP), and nuclear localization signal (NLS) into the NotI and Article Title: SLIRP maintains energy metabolism homeostasis in colorectal cancer by stabilizing mitochondrial-encoded mRNAs. Article Snippet: BACKGROUND: Colorectal cancer (CRC) is a highly vascularised tumour often characterised by elevated oxidative phosphorylation (OXPHOS) activity, positioning OXPHOS as a potential metabolic vulnerability for targeted therapy.. SLIRP is an RNA-binding protein involved in the post-transcriptional regulation of mitochondrial gene expression.. However, its specific function and underlying mechanism in CRC remain poorly understood. Article Title: Comparative Genomic Analysis Identifies FleQ and GcbB as Virulence-Associated Factors in Pseudomonas syringae pv. tabaci Strains. Article Snippet: .. For deletion constructs, these flanking fragments were first cloned into pHSG396 (TaKaRa) via HindIII and Article Title: Comparative Genomic Analysis Identifies FleQ and GcbB as Virulence‐Associated Factors in Pseudomonas syringae pv. tabaci Strains Article Snippet: .. For deletion constructs, these flanking fragments were first cloned into pHSG396 (TaKaRa) via HindIII and Article Title: METTL1 interacts with XPO5 to modulate pre-miRNA export Article Snippet: The cells were verified to be free from mycoplasma contamination using the LookOut Mycoplasma PCR Detection Kit (MP0035, Sigma–Aldrich). .. The METTL1-V5-APEX, EGFP-V5-APEX, and APEX2-NLS plasmids were constructed by inserting the coding sequence of full-length METTL1, enhanced green fluorescent protein (EGFP), and nuclear localization signal (NLS) into the NotI and Sequencing:Article Title: METTL1 interacts with XPO5 to modulate pre-miRNA export. Article Snippet: The cells were verified to be free from mycoplasma contamination using the LookOut Mycoplasma PCR Detection Kit (MP0035, Sigma–Aldrich). .. The METTL1-V5-APEX, EGFP-V5-APEX, and APEX2-NLS plasmids were constructed by inserting the coding sequence of full-length METTL1, enhanced green fluorescent protein (EGFP), and nuclear localization signal (NLS) into the NotI and Article Title: METTL1 interacts with XPO5 to modulate pre-miRNA export Article Snippet: The cells were verified to be free from mycoplasma contamination using the LookOut Mycoplasma PCR Detection Kit (MP0035, Sigma–Aldrich). .. The METTL1-V5-APEX, EGFP-V5-APEX, and APEX2-NLS plasmids were constructed by inserting the coding sequence of full-length METTL1, enhanced green fluorescent protein (EGFP), and nuclear localization signal (NLS) into the NotI and Modification:Article Title: METTL1 interacts with XPO5 to modulate pre-miRNA export. Article Snippet: The cells were verified to be free from mycoplasma contamination using the LookOut Mycoplasma PCR Detection Kit (MP0035, Sigma–Aldrich). .. The METTL1-V5-APEX, EGFP-V5-APEX, and APEX2-NLS plasmids were constructed by inserting the coding sequence of full-length METTL1, enhanced green fluorescent protein (EGFP), and nuclear localization signal (NLS) into the NotI and Article Title: METTL1 interacts with XPO5 to modulate pre-miRNA export Article Snippet: The cells were verified to be free from mycoplasma contamination using the LookOut Mycoplasma PCR Detection Kit (MP0035, Sigma–Aldrich). .. The METTL1-V5-APEX, EGFP-V5-APEX, and APEX2-NLS plasmids were constructed by inserting the coding sequence of full-length METTL1, enhanced green fluorescent protein (EGFP), and nuclear localization signal (NLS) into the NotI and Plasmid Preparation:Article Title: METTL1 interacts with XPO5 to modulate pre-miRNA export. Article Snippet: The cells were verified to be free from mycoplasma contamination using the LookOut Mycoplasma PCR Detection Kit (MP0035, Sigma–Aldrich). .. The METTL1-V5-APEX, EGFP-V5-APEX, and APEX2-NLS plasmids were constructed by inserting the coding sequence of full-length METTL1, enhanced green fluorescent protein (EGFP), and nuclear localization signal (NLS) into the NotI and Article Title: A combinatorial genetics approach reveals limits to redundancy within Plasmodium falciparum invasion ligand families Article Snippet: .. Amplified homology regions and recoded repair templates were inserted into the pCC1 plasmid (PlasmoGEM Sanger) between SpeI and Article Title: METTL1 interacts with XPO5 to modulate pre-miRNA export Article Snippet: The cells were verified to be free from mycoplasma contamination using the LookOut Mycoplasma PCR Detection Kit (MP0035, Sigma–Aldrich). .. The METTL1-V5-APEX, EGFP-V5-APEX, and APEX2-NLS plasmids were constructed by inserting the coding sequence of full-length METTL1, enhanced green fluorescent protein (EGFP), and nuclear localization signal (NLS) into the NotI and Knockdown:Article Title: SLIRP maintains energy metabolism homeostasis in colorectal cancer by stabilizing mitochondrial-encoded mRNAs. Article Snippet: BACKGROUND: Colorectal cancer (CRC) is a highly vascularised tumour often characterised by elevated oxidative phosphorylation (OXPHOS) activity, positioning OXPHOS as a potential metabolic vulnerability for targeted therapy.. SLIRP is an RNA-binding protein involved in the post-transcriptional regulation of mitochondrial gene expression.. However, its specific function and underlying mechanism in CRC remain poorly understood. Expressing:Article Title: A Wnt-induced conformational phospho-switch in DVL3 controls association with Frizzled receptors and Wnt/β-catenin signaling Article Snippet: hDVL3 segments of interest (IDR2, amino acids 335 to 396; DEP, amino acids 398 to 496; PDZ, amino acids 243 to 351; and PDZ-IDR2-DEP, amino acids 243 to 496) were amplified by polymerase chain reaction (PCR) and inserted into a pETM11 or pET-Z (IDR2) expression vectors via restriction digestion between the 5′-NcoI and 3′-KpnI sites, using BspHI (R0517, NEB) and KpnI (R3142S, NEB) restriction enzymes. .. The DNA segment encoding CK1δ or ε was amplified by PCR and inserted into pETM11 expression vector via restriction digestion between the 5′-NcoI and Clone Assay:Article Title: Comparative Genomic Analysis Identifies FleQ and GcbB as Virulence-Associated Factors in Pseudomonas syringae pv. tabaci Strains. Article Snippet: .. For deletion constructs, these flanking fragments were first cloned into pHSG396 (TaKaRa) via HindIII and Article Title: Comparative Genomic Analysis Identifies FleQ and GcbB as Virulence‐Associated Factors in Pseudomonas syringae pv. tabaci Strains Article Snippet: .. For deletion constructs, these flanking fragments were first cloned into pHSG396 (TaKaRa) via HindIII and Inverse PCR:Article Title: Comparative Genomic Analysis Identifies FleQ and GcbB as Virulence-Associated Factors in Pseudomonas syringae pv. tabaci Strains. Article Snippet: .. For deletion constructs, these flanking fragments were first cloned into pHSG396 (TaKaRa) via HindIII and Article Title: Comparative Genomic Analysis Identifies FleQ and GcbB as Virulence‐Associated Factors in Pseudomonas syringae pv. tabaci Strains Article Snippet: .. For deletion constructs, these flanking fragments were first cloned into pHSG396 (TaKaRa) via HindIII and |